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. 2020 Jul 2;12(7):717. doi: 10.3390/v12070717

Table 2.

PCR primers.

Agent Target Gene Forward Primer (5′–3′) Reverse Primer (5′–3′) Product Size (nt)
Jogalong virus NS5B CAGGTCCCTATTCTTACACGG TCTGGTAACCGAGGTGTTGC 337
Broome phasivirus 1 RdRp TTCAGATGGATTAAACCTGGCG CTAGATCTTCTTGCCACTTCAGC 269
Parry’s Creek phasivirus 1 RdRp CCAGACTGTTAGCAGCATCAATC TCAATTCCTCTTGCCTGGAGAG 225
Fitzroy Crossing tenuivirus 1 RdRp CTGGCATTGCCGGATTATCC CTAGGCTTGAAATGACTCCAGG 351
Parry’s Creek negev-like virus 1 RdRp AACTGCAGAGGGTGATATCGG ATAGCATCGCCGCTCTTCC 204
Fitzroy Crossing iflavirus 1 Polyprotein GTTGCAATACTACCAACGGCTC CAAACCCACCATCGTGGTC 240

Nt, nucleotide; RdRp, RNA-dependent RNA polymerase; NS5B, non-structural protein 5B.