Table 2.
Agent | Target Gene | Forward Primer (5′–3′) | Reverse Primer (5′–3′) | Product Size (nt) |
---|---|---|---|---|
Jogalong virus | NS5B | CAGGTCCCTATTCTTACACGG | TCTGGTAACCGAGGTGTTGC | 337 |
Broome phasivirus 1 | RdRp | TTCAGATGGATTAAACCTGGCG | CTAGATCTTCTTGCCACTTCAGC | 269 |
Parry’s Creek phasivirus 1 | RdRp | CCAGACTGTTAGCAGCATCAATC | TCAATTCCTCTTGCCTGGAGAG | 225 |
Fitzroy Crossing tenuivirus 1 | RdRp | CTGGCATTGCCGGATTATCC | CTAGGCTTGAAATGACTCCAGG | 351 |
Parry’s Creek negev-like virus 1 | RdRp | AACTGCAGAGGGTGATATCGG | ATAGCATCGCCGCTCTTCC | 204 |
Fitzroy Crossing iflavirus 1 | Polyprotein | GTTGCAATACTACCAACGGCTC | CAAACCCACCATCGTGGTC | 240 |
Nt, nucleotide; RdRp, RNA-dependent RNA polymerase; NS5B, non-structural protein 5B.