Table 4.

PCR screening of individual Culex annulirostris mosquitoes for viruses identified by HTS of pooled samples.

Site	Trap	Jogalong Virus	Parry’s Creek Negev-Like Virus 1	Fitzroy Crossing Iflavirus 1	Broome Phasivirus 1	Parry’s Creek Phasivirus 1	Fitzroy Crossing Tenui-Like Virus 1
Broome	B1	0/50	0/50	0/50	35/50 (70%)	0/50	0/50
	B2	0/50	1/50 (2%)	0/50	24/50 (48%)	0/50	0/50
Fitzroy Crossing	FC1	0/50	0/50	1/50 (2%)	2/50 (4%)	0/50	2/50 (4%)
	FC2	0/50	1/50 (2%)	0/50	0/50	1/50 (2%)	0/50
Parry’s Creek	PC1	1/50 (2%)	2/50 (4%)	0/50	2/50 (4%)	14/50 (28%)	0/50
	PC2	0/50	3/50 (6%)	0/50	1/50 (2%)	2/50 (4%)	0/50
Total		1/300 (0.3%)	7/300 (2.3%)	1/300 (0.3%)	64/300 (21.3%)	17/300 (5.7%)	2/300 (0.7%)