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. 2020 Jul 8;13(14):3052. doi: 10.3390/ma13143052

Figure 4.

Figure 4

Gene expression of bone-specific markers as determined by quantitative reverse-transcription polymerase chain reaction (qRT-PCR) at day 7 and 21 of culture. Graphs show the fold change of gene expression relative to the expression in the cells at day 0. Symbols indicate statistical significance vs. day 0 (+), vs. control (*), vs. PEMF (§) and vs. OF (°) as determined by two-way ANOVA (N = 2, n = 3). Abbreviations: OSX, osterix; Runx-2, runt-related transcription factor-2; ALP, alkaline phosphatase; OSC, osteocalcin; DCN, decorin; COL-I, type-I collagen.