Figure 2.

(A) The experimental procedure of diluting infected or cells transfected with viral RNA into a background of untreated cells and pancreas extract. (B) Comparison of recovery of three forms of viral RNA from in pancreas extract treatment. Intact CVB3 virions, CVB3-infected cells, or TD-CVB3 transfected cells across a range of dilutions of infected/transfected cells in a background of untreated cells were treated with pancreatic extract before RNA extraction and recovery. RT-PCR results are converted from Ct (threshold cycle) to genomes using a standard curve established with known quantities of viral RNA. RT-PCR assay with SYBR green has an effect on endpoint Ct, which, when converted on standard curves (dashed line), indicates an effective lowest level of detection in assay. Cell Cont represents assay results from untreated cells. The calculated input of virus genomes in each experiment is shown in white bars. The red arrows indicate expected loss of virus signal from virus not adsorbed by cells during the 1-h infection incubation and washing step.