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. 2020 Jun 28;12(7):699. doi: 10.3390/v12070699

Figure 4.

Figure 4

Analysis of the impact of SS-derived fractions on FCV and MNV proteins and genomes. (A, B) DMSO and SS-derived fractions were added to solutions containing purified FCV and the reactions proceeded at 25 °C for (A) 18 h followed by SDS–PAGE and CBB staining and (B) 12 h followed by RT–PCR using FCV-Primer set which amplifies 264 bp region on FCV gene. M: Marker, Aqu: Aqueous extract. (C,D) DMSO and SS-derived fractions were added to solutions containing purified MNV and the reactions proceeded at 25 °C for 48 h followed by (C) WB to detect MNV VP1 proteins and (D) RT–PCR using MNV-F1 and -R1 primers which amplify 721 bp region on MNV gene.