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. 2020 Jun 29;12(7):702. doi: 10.3390/v12070702

Figure 6.

Figure 6

The ARV p10 CM restores fusion activity and clustering of MdRV p10 in plasma membrane microdomains. (A) Transfected QM5 cells with authentic ARV or MdRV p10 or expressing the recombinant MdRV/ARV p10 CM construct (MdCM) were fixed and Giemsa stained at the indicated times post-transfection, and syncytial nuclei present in five random fields from triplicate wells were quantified by bright field microscopy at 100× magnification. Results are mean ± SEM (n = 3 experiments). (B) Transfected QM5 cells expressing the p10 constructs described in panel (a) were fixed and Giemsa stained at 36 h post-transfection to detect syncytium formation by bright field microscopy at 100× magnification. (C) Transfected QM5 cells co-expressing N-terminally FLAG-tagged and N-terminally Myc-tagged versions of the indicated p10 constructs were fixed without permeabilization at 24 h post-transfection, and surface-localized p10 proteins were detected using mouse-α-FLAG and rabbit-α-Myc antisera with Alexa Fluor 488 goat anti-mouse (green) and Alexa Fluor 555 goat anti-rabbit (red) secondary antibodies. Images of the two fluorescence channels are superposed in the merged image. Boxed regions are magnified in the insets.