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. 2020 Jun 29;12(7):702. doi: 10.3390/v12070702

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The ARV p10 CM restores MdRV p10 multimerization. (A) QM5 cells were co-transfected with C-terminally EGFP- and mCherry tagged versions of the indicated p10 constructs and analyzed for sensitized emission FRET. Images from the donor and acceptor channels and the calculated normalized FRET (NFRET) image are shown. The NFRET range is denoted by color gradations. (B) Best-fit Gaussian distributions of calculated NFRET images from 10 cells in each of the two separate experiments were used to calculate mNFRET values, presented as mean ± SEM, from cells co-transfected with fluorescently tagged versions of the indicated p10 constructs.