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. 2020 Jun 30;9(7):821. doi: 10.3390/plants9070821

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Bacteria growth assay. Twenty-five-day-old Arabidopsis plants were inoculated with Pseudomonas syringae pv. tomato strain DC3000 (Pst DC3000) expressing avrRpt2 by spraying a bacterial cell suspension. Six leaf disks from each plant were collected randomly at 3 DPI, and bacterial cells were extracted and plated out on a solid selective medium containing two antibiotics. Inoculated plants were wild-type Arabidopsis (Col-0), cdr1 mutant (cdr1), and four transgenic cdr1 lines (cdr1/PtCDR2-6, cdr1/PtCDR2-9, cdr1/PtCDR8-5, and cdr1/PtCDR8-11)