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. 2020 Jun 30;9(7):821. doi: 10.3390/plants9070821

Figure 4.

Figure 4

Relative expression of PR1 in wild-type Arabidopsis (Col-0), its cdr1 mutant (cdr1), and four transgenic lines (cdr1/PtCDR2-6, cdr1/PtCDR2-9, cdr1/PtCDR8-5, and cdr1/PtCDR8-11). Relative expression of PR1 was determined using the 2−ΔΔCT method. (A) Plants were sprayed with Pseudomonas syringae pv. tomato strain DC3000 (Pst DC3000) expressing avrRpt2 at an OD600 0f 0.001 in 10 mM MgCl2 and 0.02% Silwet. Leaf samples were collected at 0 and 3 DPI and total RNA was isolated for quantitative real-time PCR analysis. (B) Control plants were sprayed with 10 mM MgCl2 and 0.02% Silwet.