Table 6.

MNA test results for 50× concentrated BAFASAL^®.

Test Item	CBPI	RI [%]	Cytotoxicity%	MN [‰]	p Value	Cells with MN [‰]	p Value	Result
3 h (−S9)
PBS control	1.97	100	0	36	NA	32	NA	NA
0.2 µg/mL MMC	1.89	92	8	52	0.0243 (*)	45	0.0366 (*)	positive
0.4 µg/mL MMC	1.91	93.7	6.3	70	<0.0001 (****)	64	0.0001 (****)	positive
H2O control	1.95	100	0	31	NA	28	NA	NA
0.25 µL/mL BAFASAL® 50×	1.97	102.2	−2.2	32	0.9279	31	0.9243	negative
0.5 µL/mL BAFASAL® 50×	1.98	103.8	−3.8	26	0.9448	25	0.7269	negative
1 µL/mL BAFASAL® 50×	1.99	104.5	−4.5	28	0.3837	24	0.6028	negative
2 µL/mL BAFASAL® 50×	2.03	109.3	−9.3	33	0.6002	30	0.5204	negative
5 µL/mL BAFASAL® 50×	2.06	11.9	−11.9	32	0.7561	29	0.8099	negative
27 h (−S9)
PBS control	1.83	100	0	9	NA	9	NA	NA
0.1 µg/mL MMC	1.78	94.9	5.1	21	0.0033 (**)	20	0.0039 (***)	positive
0.2 µg/mL MMC	1.83	100.8	−0.8	26	<0.0001 (****)	24	0.0002 (***)	positive
H2O control	1.82	100	0	12	NA	12	NA	NA
0.25 µL/mL BAFASAL® 50×	1.85	104.4	−4.4	17	0.8846	15	0.8846	negative
0.5 µL/mL BAFASAL® 50×	1.85	104.7	−4.7	14	0.2936	13	0.5003	negative
1 µL/mL BAFASAL® 50×	1.79	96.4	3.6	14	0.6833	13	0.8924	negative
2 µL/mL BAFASAL® 50×	1.84	102.9	−2.9	16	0.6807	14	0.8896	negative
5 µL/mL BAFASAL® 50×	1.87	106	−6	12	0.3558	11	0.5889	negative
3 h (+S9)
PBS control	1.86	100	0	38	NA	35	NA	NA
5 µg/mL CP	1.74	86.7	13.3	91	<0.0001 (****)	86	<0.0001 (****)	positive
10 µg/mL CP	1.64	74.6	25.4	152	<0.0001 (****)	143	<0.0001 (****)	positive
H2O control	1.84	100	0	39	NA	35	NA	NA
0.25 µL/mL BAFASAL® 50×	1.84	99.7	0.3	40	0.9354	37	0.932	negative
0.5 µL/mL BAFASAL® 50×	1.85	101	−1	35	0.8947	32	0.7669	negative
1 µL/mL BAFASAL® 50×	1.92	109.2	−9.2	31	0.5972	29	0.7181	negative
2 µL/mL BAFASAL® 50×	1.84	99.5	0.5	40	0.259	39	0.3324	negative
5 µL/mL BAFASAL® 50×	1.92	110.1	−10.1	39	0.8891	36	0.5846	negative

Notes: Statistically significant levels: ns p > 0.05; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p < 0.0001.