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The currently used high-performance liquid chromatography (HPLC) method utilises an isocratic mixture of 90% 59-mM TBAP/10% MeOH (1.2 mL/min). The chromatogram shows the result of a [68Ga]Ga-DOTA-ZOL synthesis (complexation yield determined with radio thin-layer chromatography (TLC) was 98%). The first peak refers to all injected activity measured before the column. The ratio between both peaks is nearly 50:50.
