Table 1.
Cytokine | Forward Primer | Reverse Primer | Annealing Temp °C |
---|---|---|---|
TNF-α | AGACCCCAGCACCCAGGACTCG | GGAGATGCCATCTGTGTGAGTG | 55 |
IL-1α | GATGCCTGAGACACCCAA | GAAAGTCAGTGATCGAGGG | 53 |
IL-1β | CAAGGAGAGGAAAGAGACA | TGAGAAGTGCTGATGTACCA | 53 |
IL-6 | TCCAGAACGAGTATGAGG | CATCCGAATAGCTCTCAG | 52 |
β-actin | CGCACCACTGGCATTGTC | TCCAAGGCGACGTAGCAG | 55 |
IFN-α | ACACACACCTGGT | GATGACAGCAGAAATGA | 52 |
IFN-β | RTCTGSAGCCAAT | CAGGCACACCTGT | 52 |
IFN-γ | ATAACCAGGTCATTCAAAGG | ATTCTGACTTCTCTTCCGCT | 55 |
IL-8 | TGGGCCACACTGTGAAAAT | TCATGGATCTTGCTTCTCAGC | 53 |
IL-10 | TGCTGGATGACTTTAAGGG | AGGGCAGAAAGCGATGACA | 53 |
IL-12 | GAGGCCTGTTTACCACTGGA | CTCATAGATACTTCTAAGGCACAG | 58 |
IL-4 | TGCATTGTTAGCGTCTCCTG | AGGTCTTTCAGCGTACTTGT | 56 |