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. 2020 Jun 29;12(7):701. doi: 10.3390/v12070701

Table 1.

The set of primers used for quantifications of apoptosis-related cytokine mRNA.

Cytokine Forward Primer Reverse Primer Annealing Temp °C
TNF-α AGACCCCAGCACCCAGGACTCG GGAGATGCCATCTGTGTGAGTG 55
IL-1α GATGCCTGAGACACCCAA GAAAGTCAGTGATCGAGGG 53
IL-1β CAAGGAGAGGAAAGAGACA TGAGAAGTGCTGATGTACCA 53
IL-6 TCCAGAACGAGTATGAGG CATCCGAATAGCTCTCAG 52
β-actin CGCACCACTGGCATTGTC TCCAAGGCGACGTAGCAG 55
IFN-α ACACACACCTGGT GATGACAGCAGAAATGA 52
IFN-β RTCTGSAGCCAAT CAGGCACACCTGT 52
IFN-γ ATAACCAGGTCATTCAAAGG ATTCTGACTTCTCTTCCGCT 55
IL-8 TGGGCCACACTGTGAAAAT TCATGGATCTTGCTTCTCAGC 53
IL-10 TGCTGGATGACTTTAAGGG AGGGCAGAAAGCGATGACA 53
IL-12 GAGGCCTGTTTACCACTGGA CTCATAGATACTTCTAAGGCACAG 58
IL-4 TGCATTGTTAGCGTCTCCTG AGGTCTTTCAGCGTACTTGT 56