Figure 2.

Adenovirus dodecahedron formation and internalization. (a) Z-series of Hela cells infected for 16 h by wt-HAdV3. Nuclei are stained in red and the penton base is detected in green. The penton base is synthesized in the cytoplasm and transported to the nucleus (yellow results from superposition of the red and green signals) where dodecahedron assembly takes place. (b) Electron microscopy images of the purified adenovirus and (Pt-Dd) ‘penton dodecahedrons’ (bars: 90 and 30 nm, respectively). (c) Hela cells incubated with recombinant Pt-Dd for 1 h. Cell shapes were observed by DIC, nuclei are stained in blue, and Pt-Dd are detected in green.