Figure 3.

Lignification in the outer part of roots in accession W2165 of O. glumaepatula and rice (cv. Nipponbare) under aerated or stagnant conditions. Lignin deposits were observed in the basal parts (95–105 mm from the root apex; (a,d,g,j), middle parts (35–45 mm from the root apex; (b,e,h,k) and root tips (2.5–7.5 mm from the root apex; (c,f,i,l) of adventitious roots of 115–120 mm length. Lignin is indicated as red fluorescence with Basic Fuchsin (red arrowhead, mean of intensity value > 110.0 μm⁻²). Plants were grown in aerated nutrient solution for eight days and then transferred to deoxygenated stagnant 0.1% agar solution or continued aerated solution for 13–15 days. CP, cortical parenchyma; epi, epidermis; exo, exodermis; scl, sclerenchyma. Scale bars: 100 μm. (m) Fluorescence intensity of Basic Fuchsin at the sclerenchyma under aerated (white box) or stagnant (grey box) conditions. Black dots indicate each raw value. n = 2–4.