Figure 6.
The structural and accessory genes from M41-CK cannot rescue Beau-R replication at 41 °C. Primary CK cells seeded in 6 well plates were inoculated with (a) 104 PFU (MOI ~0.001) of Beau-R or BeauR-M41(S) and (b) 105 PFU (MOI ~0.01) of either Beau-R or BeauR-M41-Struct and incubated at either 37 °C or 41 °C. Supernatants were harvested at 24 h intervals the quantity of infectious progeny determined via titration in CK cells. Each point represents the mean of three independent experiments (n = 3) with error bars representing SEM. Statistical differences were assessed using a two-way ANOVA followed by a Tukey test for multiple comparisons. Differences are highlighted by **** (p < 0.0001) (a) between Beau-R at 37 °C and Beau-R 41 °C, as well as BeauR-M41(S) at 37 °C and 41 °C and (b) between Beau-R at 37 °C and Beau-R at 41 °C as well as BeauR-M41-Struct at 37 °C and BeauR-M41-Struct at 41 °C.