Table 2.

Showing the prevalence of S protein epi in other HBV genotypes (B–I).

Epitope Sites in S Protein	AA Sequence	B (n = 2905)	C (n = 5575)	E (n = 1118)	F (n = 477)	G (n = 86)	H (n = 69)	I (n = 78)	Prevalence (%) = 1 − $\left[\frac{Count of Seq}{10308}\right]$ *%	Degree of Conservation ↓ (+: Variable)	epi Previously Discussed
17–31	AFGKFLWEWASARFS	E = 998	C = 32	F = 1					10,0	+	[42]
180–194	AGFFLLTRILTIPQS	B = 62	C = 4627	E = 5	F = 2	G = 75			46,3	++	[43]
90–104	CLIFLLVLLDYQGML	B = 2605	C = 4661	E = 1034	F = 442	G = 84	H = 65	I = 70	86,9	++++	[44]
69–83	CPGYRWMCLRRFIIF	B = 2384	C = 5064	E = 1027	F = 465	G = 84	H = 67	I = 65	88,8	++++	[42]
19–33	FFLLTRILTIPQSLD	B = 63	C = 4717	E = 5	F = 2	G = 76			47,2	++	[45,46,47]
158–172	FGKFLWEWASARFSW	E = 996	C = 31	F = 1					10,0	+	[48]
20–34	FLLTRILTIPQSLDS	B = 63	C = 4709	E = 5	F = 5	G = 78			47,1	++	[49]
93–107	FLLVLLDYQGMLPVC	B = 2616	C = 4720	E = 1031	F = 446	G = 84	H = 66	I = 72	87,7	++++	[50]
161–175	FLWEWASARFSWLSL	B = 1	E = 997	C = 81	F = 3	I = 16			10,7	+	[42]
179–193	FVQWFVGLSPTVWLS	B = 2630	C = 4698	E = 76	G = 85	I = 75			73,4	+++	[51]
18–32	GFFLLTRILTIPQSL	B = 63	C = 4618	E = 5	F = 2	G = 76			46,2	++	-
159–173	GKFLWEWASARFSWL	E = 1002	C = 31	F = 3					10,1	+	-
202–216	GPSLYSILSPFLPLL	C = 19	E = 2						0,2	+	[48]
71–85	GYRWMCLRRFIIFLF	B = 82	C = 5065	E = 1032	F = 464	G = 84	H = 67	I = 65	66,5	+++	-
92–106	IFLLVLLDYQGMLPV	B = 2606	C = 4659	E = 1030	F = 443	G = 84	H = 66	I = 70	86,9	++++	-
195–209	IWMMWYWGPSLYSIL	B = 3	C = 24	E = 2					0,3	+	-
160–174	KFLWEWASARFSWLS	B = 1	E = 1012	C = 34	F = 3	I = 16			10,3	+	-
91–105	LIFLLVLLDYQGMLP	B = 2611	C = 4656	E = 1031	F = 443	G = 84	H = 66	I = 70	86,9	++++	[52]
21–35	LLTRILTIPQSLDSW	B = 63	C = 4754	E = 5	F = 5	G = 77			47,6	++	[53]
94–108	LLVLLDYQGMLPVCP	B = 2653	C = 4729	E = 1032	F = 445	G = 84	H = 66	I = 72	88,1	++++	[54]
15–29	LQAGFFLLTRILTIP	B = 62	C = 4735	E = 5	F = 2	G = 76			47,3	++	[54]
192–206	LSVIWMMWYWGPSLY	B = 772	C = 4249	E = 889	G = 1	I = 50			57,8	++	[44]
95–109	LVLLDYQGMLPVCPL	B = 2619	C = 4708	E = 1022	F = 444	G = 84	H = 66	I = 73	87,5	++++	[55,56]
162–176	LWEWASARFSWLSLL	B = 7	E = 1001	C = 88	F = 445	G = 2	H = 65	I = 75	16,3	+	[54]
205–219	LYSILSPFLPLLPIF	C = 21	E = 2						0,2	+	-
178–192	PFVQWFVGLSPTVWL	B = 2649	C = 4723	E = 76	G = 85	I = 74			73,8	+++	-
70–84	PGYRWMCLRRFIIFL	B = 2375	C = 5117	E = 1033	F = 466	G = 85	H = 67	I = 65	89,3	++++	[49]
66–80	PPTCPGYRWMCLRRF	B = 60	C = 751	E = 14	F = 464	G = 77	H = 68		13,9	+	[44]
203–217	PSLYSILSPFLPLLP	C = 19	E = 2						0,2	+	-
67–81	PTCPGYRWMCLRRFI	B = 60	C = 750	E = 14	F = 464	G = 77	H = 68		13,9	+	[57]
16–30	QAGFFLLTRILTIPQ	B = 62	C = 4645	E = 5	F = 2	G = 76			46,5	++	[58]
181–195	QWFVGLSPTVWLSVI	B = 2588	C = 4310	E = 73	G = 6	I = 71			68,4	+++	[58]
38–52	SLNFLGGTTVCLGQN	B = 5	C = 2	E = 2					0,1	+	[55,56,57]
204–218	SLYSILSPFLPLLPI	C = 19	E = 2						0,2	+	[44]
193–207	SVIWMMWYWGPSLYS	B = 3	C = 24	E = 2					0,3	+	-
155–169	SWAFGKFLWEWASAR	E = 998	C = 31	F = 1					10,0	+	-
68–82	TCPGYRWMCLRRFII	B = 63	C = 757	E = 17	F = 466	G = 77	H = 68		14,0	+	-
37–51	TSLNFLGGTTVCLGQ	B = 5	C = 2	E = 2					0,1	+	[59,60]
194–208	VIWMMWYWGPSLYSI	B = 3	C = 24	E = 2					0,3	+	-
14–28	VLQAGFFLLTRILTI	B = 61	C = 4676	E = 5	F = 2	G = 76			46,8	++	[61]*
180–194	VQWFVGLSPTVWLSV	B = 2636	C = 4584	E = 77	G = 6	I = 75			71,6	+++	[62]
156–170	WAFGKFLWEWASARF	E = 1000	C = 32	F = 1					10,0	+	[44]
163–177	WEWASARFSWLSLLV	B = 7	E = 1002	C = 87	F = 445	G = 2	H = 64	I = 75	16,3	+	-
182–196	WFVGLSPTVWLSVIW	B = 2506	C = 4125	E = 73	G = 6	I = 71			65,8	+++	[63]
201–215	WGPSLYSILSPFLPL	C = 19	E = 2						0,2	+	[52]
196–210	WMMWYWGPSLYSILS	B = 3	C = 25	E = 2					0,3	+	-
36–50	WTSLNFLGGTTVCLG	B = 5	C = 2	E = 2					0,1	+	-
35–49	WWTSLNFLGGTTVCL	B = 5	C = 2	E = 2					0,1	+	-
199–213	WYWGPSLYSILSPFL	C = 18	E = 2						0,2	+	-
206–220	YSILSPFLPLLPIFF	C = 19	E = 2						0,2	+	-
200–214	YWGPSLYSILSPFLP	C = 18	E = 2						0,2	+	-

+ The degree of conservation. The scale used: if score > = 100, then highly conserved and will be denoted by ‘+++++’. elif score > = 85: then semi conserved = ‘++++’. elif score > = 60: region of mutation and is denoted by ‘+++’. elif score > = 20, then highly variable region = ‘++’. else: high escape mutation = ‘+’. n represents the number of sequences used in the analysis. B represents full-length genotype B sequences, C represents full-length genotype C sequences, etc. 17–31 representing the beginning the position occupied by the 14-mer epitope predicts (e.g., 17 is the first amino acid of the epitope, while 31 is the last amino acid of the epitope).