. 2020 Jul 13;12(7):752. doi: 10.3390/v12070752

Table 2.

List of oligonucleotides and PCR conditions used in this study.

Virus	Target Region	Primer Name	Sequence 5′–3′	Amplicon Length (bp)	PCR Condition	Reference
Pestivirus	5′UTR	324	ATGCCCWTAGTAGGACTAGCA	288	95 °C × 2 min 40 cycles (95 °C × 45 s, 52 °C × 45 s, 72 °C × 45 s) 72 °C × 5 min	[11]
Pestivirus	5′UTR	326	WCAACTCCATGTGCCATGTAC	288		[11]
Simbu Serogroup	Segment S	Uni-S-59F	GATGWCCWCAACGGAAT	215	95 °C × 2 min 40 cycles (95 °C × 45 s, 55 °C × 45 s, 72 °C × 45 s) 72 °C × 5 min	[12]
Simbu Serogroup	Segment S	Uni-S-254R	TGGGGAAAATGGTTATTAAC	215		[12]
BEFV	Glucoprotein G	GF	ATGTTCAAGGTCCTCATAATTACC	1871	95 °C × 2 min 40 cycles (95°C × 45 s, 52 °C × 45 s, 72 °C × 2 min) 72 °C × 5 min	[13]
BEFV	Glucoprotein G	GR	TAATGATCAAAGAACCTATCATCA	1871		[13]
EHDV	NS3	NS3F	CAGCGCYWTATWCGATATTG	533	95 °C × 2 min 40 cycles (95 °C × 45 s, 55 °C × 45 s, 72 °C × 60 s) 72 °C × 5 min	[14]
EHDV	NS3	NS3R	TCCGGAGATACCTCCATTAC	533		[14]