Table 2.
Mass spectral characteristics and tentative identification of compounds present in 70% ethanolic leaves extract of A. elliptica.
| Peak No. | Retention Time, Min | Exact Mass | Deprotonated Molecule [M − H]− (m/z) |
Delta | MS/MS Fragment Ions | Tentative Identification | Molecular Formula |
|---|---|---|---|---|---|---|---|
| 1 | 1.00 | 192.0197 | 191.0184 | 0.0013 | 173.0080, 129.0180, 111.0074, 87.0073 | Citric acid | C6H8O7 |
| 2 | 1.19 | 332.0671 | 331.0662 | 0.0009 | 312.1069, 271.0454, 241.0342, 211.0238, 169.0130 | Monogalloylglucose | C13H16O10 |
| 3 | 3.14 | 452.4087 | 451.3394 | 0.0693 | 302.5710, 289.0708, 210.2854, 151.2638 | (+)-Catechin 6-C-glucoside | C21H24O11 |
| 4 | 3.31 | 282.0817 | 281.0331 | 0.0486 | 239.1548, 219.1387, 207.1384, 201.1277, 165.0904 | 5,7-Dimethoxyflavone | C17H14O4 |
| 5 | 3.40 | 282.0817 | 281.0329 | 0.0488 | 239.1548, 219.1387, 207.1384, 201.1277, 165.0904 | 5,7-Dimethoxyflavone isomer | C17H14O4 |
| 6 | 3.45 | 282.0817 | 281.0328 | 0.0489 | 239.1548, 219.1387, 207.1384, 201.1277, 165.0904 | 5,7-Dimethoxyflavone isomer | C17H14O4 |
| 7 | 3.70 | 762.1571 | 761.1343 | 0.0228 | 610.1257, 301.0352, 169.0131 | Quercetin 3-O-(2″-O-galloyl)-rutinoside | C34H34O20 |
| 8 | 3.80 | 550.1402 | 549.1448 | 0.0046 | 429.1028, 369.0819, 339.0715, 309.0611, 269.0662 | Formononetin 7-O-(2″-p-hydroxybenzoylglucoside) | C29H26O11 |
| 9 | 4.14 | 463.1168 | 461.1610 * | 0.0442 | 314.0427, 300.1083, 287.0562, 255.0286 | Oxycoccicyanin/Peonidin-3-glucoside | C22H23O11 |
| 10 | 4.34 | 454.1555 | 453.1605 | 0.0050 | 386.9793, 367.0700, 301.0338, 284.0323, 176.0435 | KB-2/5,2″,4′,5′-Tetrahydroxy-3-(3-hydroxy-3-methylbutyl)-6″,6″-dimethylpyrano[2″,3″:7,8]flavone | C25H26O8 |
| 11 | 4.57 | 914.1469 | 913.1451 | 0.0018 | 761.1329, 743.1249, 609.1331, 591.1135, 575.0852, 453.0853 | Theasinensin A | C44H34O22 |
| 12 | 4.79 | 350.0024 | 349.0591 | 0.0567 | 269.6351, 241.0011, 227.0375, 152.0433 | Apigenin 7-sulfate | C15H10O8S |
| 13 | 4.95 | 290.0790 | 289.1802 | 0.1012 | 245.0812, 203.0703, 179.0335, 137.0230, 123.0437 | Catechin | C15H14O6 |
| 14 | 5.02 | 458.0776 | 457.0766 | 0.0010 | 331.0454, 305.0666, 287.0562, 269.0456, 193.0132, 169.0131 | (−)-epigallocatechin-3-gallate | C22H18O11 |
| 15 | 5.61 | 756.2040 | 755.2025 | 0.0015 | 489.1044, 301.0315, 300.0270, 271.0243, 255.0294, 178.9978 | Quercetin 3-O-(2,6-di-O-rhamnosylglucoside) | C33H40O20 |
| 16 | 5.71 | 480.0831 | 479.0816 | 0.0015 | 316.0219, 287.0189, 271.0241, 178.9979, 151.0025 | Myricetin-3-O-glucoside | C21H20O13 |
| 17 | 5.76 | 626.1410 | 625.1396 | 0.0014 | 478.0751, 317.0288, 316.0212, 271.0243, 178.9976 | Myricetin-3-O-rutinoside | C27H30O17 |
| 18 | 5.93 | 596.1305 | 595.1293 | 0.0012 | 463.0802, 301.0349, 300.0271, 283.0230, 271.0244, 178.9975 | Quercetin 3-lathyroside | C26H28O16 |
| 19 | 6.11 | 450.0726 | 449.0713 | 0.0013 | 316.0220, 287.0198, 271.0241, 178.9975 | Myricetin-3-O-arabinoside | C20H18O12 |
| 20 | 6.32 | 610.1461 | 609.1451 | 0.0010 | 463.0797, 447.0925, 301.0349, 300.0247 | Quercetin 3-O-α-l-rhamnoside-7-O-β-d-glucoside | C27H30O16 |
| 21 | 6.38 | 610.1461 | 609.1450 | 0.0011 | 301.0339, 300.0269, 271.0244, 178.9973, 151.0026 | Quercetin-3-O-rutinoside (Rutin) | C27H30O16 |
| 22 | 6.45 | 464.0882 | 463.0869 | 0.0013 | 316.0218, 287.0193, 178.9977, 151.0026 | Myricetin-3-O-rhamnoside | C21H20O12 |
| 23 | 6.47 | 381.9922 | 380.9911 | 0.0011 | 301.0349, 300.0247, 283.0245, 271.0238, 257.0445, 229.0496, 193.0133 | Quercetin 3-O-sulfate | C15H10O10S |
| 24 | 6.66 | 464.0882 | 463.0875 | 0.0007 | 301.0339, 300.0270, 271.0244, 255.0293, 178.9976, 151.0024 | Quercetin-3-O-glucoside | C21H20O12 |
| 25 | 6.95 | 434.0776 | 433.0768 | 0.0008 | 301.0346, 300.0271, 271.0243, 255.0291, 178.9982, 151.0023 | Quercetin-3-O-arabinoside | C20H18O11 |
| 26 | 7.18 | 286.0405 | 285.0395 | 0.0012 | 257.0450, 213.0545, 187.0391, 163.0021 | Kaempferol | C15H10O6 |
| 27 | 7.19 | 365.9973 | 364.9961 | 0.0010 | 285.0400, 267.0294, 255.0291, 241.0501, 229.0500, 213.0548, 178.4121, 133.0279, 105.6355 | Luteolin 7-sulfate | C15H10O9S |
| 28 | 7.44 | 448.0933 | 447.0917 | 0.0016 | 301.0339, 300.0271, 271.0243, 255.0291, 178.9975, 151.0024 | Quercetin-3-O-rhamnoside | C21H20O11 |
| 29 | 7.64 | 416.1035 | 415.1962 | 0.0927 | 252.1096, 238.9105, 177.2131, 123.0804 | 7,2′-Dihydroxyflavone 7-glucoside | C21H20O9 |
| 30 | 8.31 | 432.0984 | 431.0970 | 0.0014 | 285.0393, 284.0321, 255.0293, 227.0341 | Kaempferol-3-O-rhamnoside | C21H20O10 |
| 31 | 8.66 | 458.0776 | 457.0766 | 0.0010 | 331.0454, 305.0666, 287.0562, 269.0456, 193.0132, 169.0131 | Epigallocatechin-3-gallate isomer | C22H18O11 |
| 32 | 9.15 | 396.0079 | 395.0064 | 0.0015 | 315.0607, 272.0317, 259.0608, 151.0027 | Persicarin/Isorhamnetin 3-sulfate | C16H12O10S |
| 33 | 9.57 | 302.0354 | 301.0348 | 0.0006 | 273.0403, 178.9974, 151.0024, 121.0281 | Quercetin | C15H10O7 |
| 34 | 10.00 | 380.0129 | 379.0117 | 0.0012 | 299.0555, 284.0321, 257.0403, 243.0656, 228.0399, 211.0385, 162.5436, 151.0027, 110.0001 | Rhamnocitrin 3-O-sulfate | C16H12O9S |
| 35 | 10.37 | 542.0658 | 541.0644 | 0.0014 | 461.1082, 314.0426, 299.0188, 271.0243, 256.0363, 158.7938 | Isoscutellarein 4′-methyl ether 8-(2′-sulfatoglucoside) | C22H22O14S |
| 36 | 11.20 | 328.2177 | 327.2171 | 0.0006 | 229.1440, 211.1331, 171.1015 | Trihydroxy octadecadienoic acid | C18H32O5 |
| 37 | 11.98 | 328.2177 | 327.2171 | 0.0006 | 229.1440, 211.1331, 171.1015 | Trihydroxy octadecadienoic acid isomer | C18H32O5 |
| 38 | 13.78 | 376.2541 | 375.1778 | 0.0763 | 333.6364, 330.1770, 329.1730, 307.1919, 235.1334, 207.0993 | Ardisiphenol B | C23H36O4 |
| 39 | 14.49 | 290.2173 | 289.1803 | 0.0370 | 245.1902, 161.9148, 148.7701, 123.0794 | Ardisinol II | C19H30O2 |
| 40 | 16.00 | 884.5061 | 883.4165 | 0.0896 | 837.4141, 559.1864, 456.2514, 397.1332, 277.2172 | Ardisianoside D | C46H76O16 |
| 41 | 16.37 | 346.2497 | 345.1830 | 0.0667 | 306.9802, 292.2949, 192.5377 | Ardisianone A | C22H34O3 |
| 42 | 16.54 | 722.1410 | 721.3632 | 0.2222 | 675.3585, 569.1615, 415.1444, 400.9850, 305.0875, 277.2165 | Thonningianin B | C35H30O17 |
| 43 | 16.96 | 722.1410 | 721.3631 | 0.2221 | 675.3580, 569.1614, 415.1447, 400.9853, 305.0875, 277.2166 | Thonningianin B isomer | C35H30O17 |
| 44 | 18.14 | 294.1758 | 293.2114 | 0.0356 | 275.2011, 155.1072, 141.1270, 127.1115, 121. 1009 | Embelin | C17H26O4 |
| 45 | 18.67 | 560.0399 | 559.1269 | 0.0870 | 354.6981, 286.8783, 228.8837, 121.6282 | Gossypetin 8-glucoside-3-sulfate | C21H20O16S |
| 46 | 19.33 | 426.3789 | 425.2304 | 0.1485 | 271.0612, 245.0811, 203.0705, 177.0179, 151.0386 | Friedelan-3-one | C30H50O |
| 47 | 20.00 | 572.2621 | 571.2880 | 0.0259 | 530.8586, 487.1684, 391.2236, 255.2324, 241.0111, 223.0012 | Ardisiaquinone G | C31H40O10 |
| 48 | 22.76 | 378.2334 | 377.2329 | 0.0005 | 359.2229, 335.2515, 316.2326, 152.0106 | Cornudentanone | C22H34O5 |
| 49 | 23.12 | 324.0328 | 323.1162 | 0.0834 | 279.2323, 265.8335, 216.0093, 184.0194 | 6-Chlorocatechin | C15H13ClO6 |
| 50 | 23.80 | 360.0772 | 359.0612 | 0.0160 | 317.2494, 315.2661, 245.0694, 211.2592 | Acerosin | C18H16O8 |
| 51 | 24.48 | 386.2093 | 385.2741 | 0.0648 | 268.6892, 176.3903, 153.4738 | Ardisinone E | C23H30O5 |
| 52 | 25.02 | 318.2558 | 317.2477 | 0.0081 | 300.0236, 231.3264, 192.0054, 178.9974, 151.0025 | Bilobol | C21H34O2 |
| 53 | 25.24 | 502.1919 | 501.3211 | 0.1292 | 486.2979, 473.2810, 456.2825, 443.2783, 435.2527 | Cycloheterophyllin | C30H30O7 |
| 54 | 26.18 | 418.3083 | 417.2634 | 0.0449 | 401.6784, 375.2524, 335.2505, 308.5951, 193.0859 | Maesaquinone | C26H42O4 |
| 55 | 26.33 | 332.1551 | 331.2267 | 0.0716 | 313.2373, 287.2391, 254.8929, 225.6182, 213.1681 | Gibberellin A4 | C19H24O5 |
| 56 | 26.54 | 336.1163 | 335.1342 | 0.0179 | 332.2440, 317.2474, 305.2462, 279.2698, 230.0207 | Berberine | C20H18NO4 |
| 57 | 26.80 | 542.2879 | 541.3524 | 0.0645 | 526.3293, 511.3054, 493.2956, 359.2590, 183.6019 | Ardisiaquinone D | C31H42O8 |
| 58 | 26.93 | 426.3861 | 425.2300 | 0.1489 | 407.0769, 381.0991, 339.0862, 257.0452, 216.0410, 167.2586 | Alpha-amyrin | C30H50O |
| 59 | 27.05 | 528.2723 | 527.3369 | 0.0646 | 514.3290, 499.3061, 191.0708, 165.0543, 151.0386 | Ardisiaquinone A | C30H40O8 |
| 60 | 27.08 | 530.2952 | 529.3524 | 0.0572 | 514.3292, 499.3058, 481.2948, 453.2976, 347.2579, 225.7453 | Ardisiaquinone J | C30H42O8 |
| 61 | 28.09 | 334.1555 | 333.2426 | 0.0871 | 279.2678, 186.7317, 134.0364 | 1,5-Dibutyl methyl hydroxycitrate | C15H26O8 |
| 62 | 28.37 | 496.2824 | 495.2625 | 0.0199 | 316.1812, 278.8960, 205.1232, 181.0499, 169.0134 | Ardisenone | C30H40O6 |
* Represented [M − 2H]− ion was observed.