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Optimization conditions for β-lactoglobulin (β-Lg) detection using the electrochemical assay: (A,B) the titration volume (2, 4, 6, 8, or 10 μL) of PEI-rGO-AuNCs used to modify the electrode; (C,D) the incubation time (10, 15, 25, 35, 45, or 55 min) of the PEI-rGO-AuNCs electrode with β-Lg standards (0.025 ng/mL).
