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Isolation and purification of active compounds. (a) The active fraction 3 was subjected to LC-MS. The significant component is at 12.9 m in the LC; (b) the exact m/z from the significant component was 237.1466 ([M+H]+, C14H21O3; calculated 237.1485), 219.1360 [M+H-H2O]+, 173.1306 [M+H-2×H2O-CO]+.
