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. 2020 Jul 1;25(13):3018. doi: 10.3390/molecules25133018

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Identification of the active compound. (a) The high-resolution mass spectrometry of the HPLC purified compound with a proton (236.1403 + 1.0078 = 237.1481). (b) Four purified and identified compounds from A. aculeatus from other research labs [12,14]. (c) The transmission-blocking activities of four compounds. (d) The transmission-blocking activity of serial dilution of asperaculane B. N: # of mosquitoes for each treatment; μ: the mean oocysts per midgut; PR: infection prevalence in mosquitoes; p: The statistical p values of each concentration comparing to the control (concentration was 0).