FIGURE 4.

MUC12 enhanced RCC cell invasion via promoting epithelial‐mesenchymal transition (EMT) process. A‐B. Knockdown of MUC12 suppressed cell invasion of 786‐O cells. A, representative invaded cells. B, statistical analysis of A. C‐D, Introduction of MUC12 into A498 cells promoted their invasive abilities. C, representative invaded cells. D, statistical analysis of C. E, Real‐time qPCR demonstrated that MUC12 reduction in 786‐O cells increased E‐cadherin and ZO‐1 while decreased other EMT‐related genes at the mRNA levels. Expression levels of genes were normalized to GAPDH mRNA levels. F, Real‐time qPCR displayed that MUC12 induction in A498 cells reduced E‐cadherin and ZO‐1 while up‐regulated other EMT related genes at the mRNA levels. Expression levels of genes were normalized to GAPDH mRNA levels. G, Western blotting confirmed contribution of MUC12 to EMT‐related markers in both 786‐O and A498 cells. GAPDH was served as loading control. H, Immunofluorescent staining of ZO1 after manipulation of MUC12 in 786‐O and A498 cells. *P < 0.05; **P < 0.01