FIGURE 6.

MUC12‐mediated RCC cell progression was dependent of c‐Jun. A, A positive correlation between c‐Jun and TGF‐β1 was observed in human RCC samples based on TCGA data set. B, Knockdown of MUC12 reduced the expression levels of c‐Jun in 786‐O cells while overexpression of MUC12 elevated c‐Jun in A498 cells. GAPDH was internal control. C, Knockdown efficiency of c‐Jun by siRNAs in 786‐O cells. GAPDH was used as loading control. D, Knockdown of c‐Jun attenuated MUC12‐mediated up‐regulation of TGF‐β1. GAPDH served as internal control. E,F, Knockdown of c‐Jun blocked MUC12 induced cell invasion of 786‐O cells. E, representative images of invaded cells. F, a statistical analysis of invaded cells. G, Knockdown of c‐Jun reduced TGF‐β1 protein levels. GAPDH was loading control. H, Knockdown of c‐Jun decreased TGF‐β1 mRNA levels. TGF‐β1 mRNA levels were normalized to GAPDH mRNA levels. *P < 0.05; **P < 0.01