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. 2020 Jul 16;12(7):767. doi: 10.3390/v12070767

Figure 2.

Figure 2

Restriction enzyme analysis of the genomes of wild-type (WT) and recombinant wild-type (rWT) viruses. (A) The genomes of CAdV1-WT and CAdV1-rWT were digested with XbaI, BamHI, and KpnI. The predicted molecular weights of the digested fragments of the viral genomes are 11250, 5551, 3383, 3301, 1955, 1834, 1587, 775, 519, 333, and 45 bp for XbaI; 13554, 10982, 5534, and 463 bp for BamHI; and 12573, 7077, 5110, 2490, 2302, and 981 bp for KpnI. M: 1-kb DNA ladder marker. (B) The genomes of CAdV2-WT and CAdV2-rWT were digested with NdeI, EcoRI, and XhoI. The predicted molecular weights of the digested fragments of the viral genomes are 14820, 9059, 4585, 1603, and 1253 bp for NdeI; 13014, 8192, 6504, and 3610 bp for EcoRI; and 12194, 7688, 7089, 2462, and 1887 bp for XhoI. M: 1-kb DNA ladder marker.