Figure 1.

Heating experiment design. Plants 28-days-old were grown on a 14-h light, 10-h dark cycle at 21 °C. The experiment began 13 h into the light phase of the cycle (1 h before the start of the dark phase), and the original light cycle continued through the experiment. Plants were subjected to one of three treatments: “Path 1” (control): 21 °C for 40 h; “Path 2”: 38 °C for 12 h, 45 °C for 4 h, and 21 °C for 24 h; or “Path 3”: 21 °C for 12 h, 45 °C for 4 h, and 21 °C for 24 h. Sampling points were at 0 h, 1 h, 12 h, 13 h, 16 h (end of heat phase for paths 2 and 3), 17 h, and 40 h. At these times, leaves 5 and 6 were removed, and the remainder of the rosette was harvested and extracted for lipid analysis. (Each plant produced one experimental sample.) The plant tray numbers (Table S1) corresponding to each treatment/time point are indicated in the yellow symbols.