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Assessment of the cytotoxic effects of juglone in J774.1 cells using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. (a) Chemical structure of juglone. (b) J774.1 cells were treated with increasing concentrations of juglone (3.1–50 μM) for 24 h, followed by quantification of cell viability using the MTT assay. Data are presented as mean ± standard deviation (SD) of three independent experiments. * p < 0.05; ** p < 0.01; vs. control.
