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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Exp Eye Res. 2019 Aug 14;187:107756. doi: 10.1016/j.exer.2019.107756

Fig. 7. Increased microglial and Muller cell activation in retinas from tunicamycin injected mice.

Fig. 7.

Wholemount retinas prepared from CX3CR1gfp/gfp mice treated with vehicle or tunicamycin (0.05 µg, 2 weeks post injection) were evaluated for microglial cells (A, B). GFAP staining of the retinal sections from control and tunicamycin injected mice (C, D). RPE/scleral flatmounts from mice treated with control or tunicamycin stained for ZO-1 (E, F). Please note the increase in microglial cell number and morphological changes indicting activation. Enhanced GFAP expression in treated mice compared to control mice demonstrate Muller glial cell activation. The smooth junctional localization and similar shape of cells demonstrated by ZO-1 staining indicates minimal changes in RPE cells of eyes injected with tunicamycin. These experiments were repeated with eyes from 5 mice with similar results. Scale bars, top panels 100 µm, middle panels 50 µm, and lower panels 20 µm.