Table 1.
Cycle of Threshold (CT) and parasite load test copy/reaction according to Real Time PCR method for macrophages before and after infection with 1 × 104 tachyzoite forms of T. gondii RH strain and control groups.
| Groups | Parasite load of macrophage cells before | Parasite load of macrophage cells after | ||
|---|---|---|---|---|
| treated with imiquimod | treated with imiquimod | |||
| Cycle of threshold for test | Parasite load test copy/reaction | Cycle of threshold for test | Parasite load test copy/reaction | |
| Control (healthy macrophages) | 30.50 | 0 | 29.76 | 0 |
| Control (infected macrophages) | 11.71 | 376435 | 10.54 | 391075 |
| Imiquimod 1 μg/ml | 18.21 | 33684 | 16.10 | 119623 |
| Imiquimod 0.1 μg/ml | 18.27 | 32500 | 16.18 | 106544 |
| Imiquimod 0.01 μg/ml | 18.51 | 29363 | 16.53 | 92671 |
| Sulfadiazine 40 μg/ml + pyrimethamine 1 μg/ml | 21.70 | 4222 | 20.80 | 18465 |
| Sulfadiazine 40 μg/ml + pyrimethamine1 μg/ml + Imiquimod 1 μg/ml | 22.20 | 10784 | 17.98 | 39608 |