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Cycle of Threshold (CT) and parasite load of Spleen Tissues according to Quantitative real time PCR (qPCR) method for all treated and control groups.
| Number | Sample type | Cycle of threshold | Parasite load test |
|---|---|---|---|
| test | for test | copy/reaction | |
| 1 | Control (healthy mice) | 30.53 | 0.04 |
| 2 | Control (infected mice) | 12.93 | 132545 |
| 3 | Imiquimod before challenge | 25.81 | 192 |
| 4 | Imiquimod after challenge | 17.21 | 14440 |
| 5 | Sulfadiazine+pyrimethamine | 28.28 | 0.22 |
| 6 | Sulfadiazine+pyrimethamine + Imiquimod | 28.15 | 0.51 |
