Table 2.

List of antibodies used for immunohistochemistry

Primary antibody	Secondary antibody
Anti-Iba-1 goat polyclonal (1:400, Abcam), 48 h, 4 °C	Anti-goat FITC conjugated (1:200, Sigma), overnight, 4 °C
Anti-GFAP mouse monoclonal (1:400, MP Biomedicals), overnight, 4 °C	Anti-mouse Cy3 conjugated (1:200, Sigma), overnight, 4 °C
Anti-ChAT goat polyclonal (1:200, Millipore), 48 h, 4 °C	Anti-goat FITC conjugated (1:200, Sigma), overnight, 4 °C
Anti-phosphorylated neurofilament mouse monoclonal (1:800, SMI), overnight, 4 °C	Anti-mouse Cy3 conjugated (1:200, Sigma), overnight, 4 °C

Iba-1 ionized calcium binding adaptor molecule 1, microglial marker, GFAP glial fibrillary acidic protein, astrocyte marker, ChAT choline acetyltransferase, motor neuron marker, FITC fluorescein isothiocyanate, Cy3 cyanine3