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. 2020 Aug 6;27:86. doi: 10.1186/s12929-020-00677-4

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Deposition of C3b and MAC on Cjr⁺-RS218 and Cjr⁻-RS218 in 40% NHS for various periods. (a) Levels of C3b deposition on Cjr⁺-RS218 and Cjr⁻-RS218 after incubation with NHS for the indicated time periods. (b) Flow cytometry histogram of C3b deposition on the bacteria after 3 h of incubation in NHS. (c) Levels of MAC deposition on Cjr⁺-RS218 and Cjr⁻-RS218 after incubation with NHS for the indicated time periods. (d) Flow cytometry histogram of MAC deposition on the bacteria after 3 h of incubation in NHS. For (a) and (c) The data are presented with geometric mean fluorescence intensity (GMFI). The HI-NHS control groups were bacteria incubated in HI-NHS, while the unstaining control groups were the bacteria without fluorescence staining. The results are shown as the means ± standard deviations, and the data are representative of three independent experiments performed in triplicate. *, P value < 0.05