Fig. 7.

Survival of the RS218 strains harboring individual genes of the cjrABC-senB gene cluster in NHS or HI-NHS and localization of CjrA. (a) and (b) The survival rates of the bacterial strains were determined after the strains were incubated for 3 h in 40% NHS (a) or 40% HI-NHS (b). The data are shown as relative survival rates compared with those of Cjr⁻-RS218. The results are shown as the means ± standard deviations, which are representative of three independent experiments performed in triplicate. (c) The localization of CjrA. The outer and inner membrane (OM and IM) fractions of CjrA-RS218, which expresses C-terminally His₆-tagged CjrA, were isolated and subjected to Western blot analysis with an anti-His₆ antibody or OmpA antiserum. OmpA serves as an OM marker. *, P value < 0.05