FIGURE 3.

CHMP4B alleviates the level of necroptosis after TBI. A, Protein levels of RIP3 and p‐MLKL obtained from TBI group and TBI + CHMP4B group. GAPDH was used as the loading control. B, Bar graphs show the results of analysis (by band density analysis) of RIP3 and p‐MLKL (n = 5 mice; data are presented as the means ± SEM). C, Immunohistochemistry of RIP3 and p‐MLKL in TBI group and TBI + CHMP4B group. (Scale bar = 50 μm.) D, Transmission electron microscopy (TEM) images of tissues. Translucent cytoplasm mitochondrial swelling and destruction of membrane integrity were observed in TBI neurons. (Scale bar = 5 or 1 μm.) *P < .05