FIGURE 6.

FOXO1 regulates CHMP4B transcription and expression. A, The relative luciferase activity in BV2 cells after co‐transfected plasmids containing transcription factors and CHMP4B. B and C, Expression of nuclear FOXO1 was detected by laser scanning confocal microscopy (scale bar = 20 μm). D, Protein level of FOXO1. GAPDH was used as the loading control. E, Bar graphs show the results of analysis (by band density analysis) of FOXO1 (n = 3; data are presented as the means ± SEM). F, qRT‐PCR analysis of FOXO1 expression in Ctrl groups and Glu groups (n = 3; data are presented as the means ± SEM). G‐L. BV2 cells were transfected with FOXO1 plasmid or siRNA for 2 d, and then added the Glu. The protein and mRNA levels of CHMP4B were determined by Western blot and qRT‐PCR (n = 3; data are presented as the means ± SEM; *P: Glu groups compared with Ctrl groups; #P: Glu + FOXO1 groups compared with the Glu + vector groups). *P < .05; **P < .01; #P < .05