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. 2020 Jul 31;8:600. doi: 10.3389/fcell.2020.00600

FIGURE 3.

FIGURE 3

Structure of EZH2 in a minimal PRC2 assembly in complex with an asymmetric di-nucleosome. The schematic at the top represents domain configuration of EZH2. The demonstrations show the same mPRC2:NCP complex from a front and back view. EZH2 domains in the structure are colored according to the linear schematic depiction. The VEFS domain of SUZ12 is colored as magenta and EED is represented as light green. H3 tail with K27M substitution of the substrate nucleosome is shown bound to the substrate groove of EZH2 SET domain (purple blue). The neighboring nucleosome with a modified H3 tail bearing a trimethylated lysine at the position of K27 (K27me3) is shown in a groove between EED and EZH2 SRM domain (teal). Zoomed demonstration of the framed areas on structure is shown at the bottom of the figure. Positively charged and polar residues of EZH2 CXC domain (solid frame) and EZH2 SBD/EED (dashed frame) within proximity to DNA back bone are labeled with their corresponding residue numbers. EZH2 SBD hydrophobic residue is shown in close proximity of DNA.