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. 2020 Jul 10;21:737–750. doi: 10.1016/j.omtn.2020.07.007

Figure 3.

Figure 3

Direct Transfer of SIRT1 from ADSCs to EPCs Using Exosome Delivery

(A) Transmission electron microscopy of exosomes from ADSCs (ADSCs-Exos group) or ADSCs overexpressing SIRT1 (ADSCs-SIRT1-Exos group). Scale bars, 100 nm. (B) Nanoparticle tracking analysis of the exosome diameters (in nanometers). (C) Expression of SIRT1 and specific exosomal surface markers (CD63 and TSG101) were detected by western blotting of ADSCs and exosomes. (D) Localization of PKH26-labeled ADSCs-Exos (red) in AMI-EPCs visualized by confocal microscopy at 0 and 24 h post-ADSCs-Exos incubation. Scale bars, 10 μm. (E) Fluorescence microscopy analysis was performed to assay the green fluorescent signals in AMI-EPCs co-cultured with ADSCs-Exos with or without GFP-labeled SIRT1 treatment. Scale bars, 10 μm.