Fig. 3.

Steady-state kinetic analysis and binding isotherm for TrCel7A enzymes on Avicel at 50 °C. The reaction mixtures were incubated for 1 h in each assay. a^convMM analysis, low enzyme concentration of 0.1 μM and high Avicel load (0–70 g/L). b^invMM, low substrate concentration of 12 g/L Avicel is saturated with enzyme concentration. Solid lines are a non-linear fit from Eqs. (1,  2). c Binding isotherm of TrCel7A wt and variants on 12 g/L Avicel at 50 °C. Solid lines represent the fitted Langmuir equation (Eq. 4). Error bars represent standard deviations from triplicate measurements