. 2020 Jul 30;7:389. doi: 10.3389/fvets.2020.00389

Table 5.

Blood serum lipid metabolites of brown egg layers fed three levels of Ca supplemented with three levels of VD₃ from 60 to 72 wk of age.

Treatments	Cho, mg/dL	Trig, mg/dL	HDL, mg/dL	vLDL, mg/dL	LDL, mg/dL	Trig: HDL Ratio	LDL: HDL Ratio	Cholesterol risk factor	Cholesterol lowering factor
Calcium level
3.5% Ca	183	150^a	57.6	29.9^a	95.7	2.62^a	1.68	1.92	3.21
4.0% Ca	180	140^b	57.6	26.6^b	95.4	2.32^b	1.67	1.89	3.14
4.5% Ca	185	133^a ^b	62.4	27.9^a ^b	95.3	2.24^b	1.54	1.95	2.99
VD₃ level
800 IU	182	142	58.5	28.3	95.5	2.43	1.64	1.91	3.13
1,000 IU	183	142	59.7	28.4	94.9	2.39	1.61	1.93	3.09
1,200 IU	183	139	58.4	27.8	95.9	2.35	1.64	1.91	3.11
Interaction Ca × VD₃ effect
3.5 800	182	149	55.6	29.7	97.1	2.69	1.76	1.89	3.30
3.5 1,000	183	154	57.9	30.7	94.4	2.68	1.64	1.94	3.17
3.5 1,200	184	147	59.3	29.4	95.7	2.49	1.64	1.93	3.14
4.0 800	182	138	58.7	27.5	95.3	2.34	1.62	1.91	3.09
4.0 1,000	179	133	56.8	26.5	95.8	2.34	1.71	1.88	3.18
4.0 1,200	178	129	57.3	25.9	95.1	2.27	1.68	1.88	3.14
4.5 800	183	139	61.2	27.7	94.1	2.26	1.55	1.94	2.99
4.5 1,000	187	140	64.5	27.9	94.8	2.17	1.49	1.98	2.93
4.5 1,200	187	141	61.6	28.2	96.9	2.29	1.58	1.92	3.04
Statistical variance
SEM	4.485	5.974	2.711	1.195	3.258	0.091	0.101	0.053	0.111
P-values
Ca	0.253	0.007	0.058	0.007	0.986	0.001	0.184	0.372	0.069
VD₃	0.971	0.844	0.857	0.844	0.941	0.550	0.931	0.867	0.911
Interaction	0.924	0.847	0.778	0.847	0.954	0.544	0.811	0.905	0.771

^a,b

Means in the same column under the same effect with different superscripts significantly different (P < 0.05). SEM. standard error of the means; Cho, cholesterol; Trig, triglycerides; HDL, high density lipoprotein; vLDL, very low density lipoprotein; LDL, low density lipoprotein; Trig:HDL ratio, triglycerlide:high density lipoprotein ratio, LDL:HDL ratio, low density lipoprotein:high density lipoprotein ratio.