. 2020 Jul 30;7:389. doi: 10.3389/fvets.2020.00389

Table 7.

Blood serum immunity parameters and some hormones of brown egg layers fed three levels of Ca supplemented with three levels of VD₃ from 60 to 72 wk of age.

Treatments	IgG, mg/dl	IgM, mg/dl	IgA, mg/dl	HIAI, Log2	HIND, Log2	Estrogen, Pg/ml	PTH, ng/ml	MDA, nmol/ml	TAC, nmol/ml	MDA/TAC Ratio	SOD, U/ml/h	CAT, U/ml/h
Calcium level
3.5% Ca	464^b	134	154^c	2.33	3.33	162^b	2.17	21.9	1.75	12.8	76.1	47.2^c
4.0% Ca	533^a	131	165^b	2.13	3.80	190^a	2.12	19.1	1.76	11.0	74.5	54.0^b
4.5% Ca	548^a	137	175^a	2.40	3.53	186^a	2.17	19.5	1.63	12.2	80.8	57.6^a
VD₃ level
800 IU	512	135	165	2.40	3.60	177	2.21	21.1	1.68	12.9	74.4	52.8^b
1,000 IU	511	135	166	2.20	3.53	173	2.09	19.9	1.66	12.1	78.6	52.5^b
1,200 IU	522	131	163	2.27	3.53	189	2.16	19.6	1.79	11.0	78.4	53.6^a
Interaction Ca × VD₃ effect
3.5 800	407	135	151	2.20	3.00	134^b	2.40	23.8	1.59	15.3	75.6	41.2^b
3.5 1,000	485	136	155	2.20	3.60	175^a ^b	2.09	21.5	1.79	12.1	75.4	51.2^a ^b
3.5 1,200	499	132	156	2.60	3.40	177^a ^b	1.99	20.4	1.85	11.1	77.2	49.3^a ^b
4.0 800	542	130	164	2.40	4.20	199^a	1.89	19.1	1.80	10.9	70.5	57.2^a
4.0 1,000	500	126	165	2.20	3.60	175^a ^b	2.17	19.6	1.71	11.6	77.9	48.4^a ^b
4.0 1,200	558	137	167	1.80	3.60	196^a	2.29	18.6	1.76	10.6	75.1	56.5^a
4.5 800	586	140	179	2.60	3.60	196^a	2.32	20.3	1.65	12.8	77.1	59.8^a
4.5 1,000	548	142	179	2.20	3.40	169^a ^b	2.02	18.5	1.49	12.6	82.4	57.9^a
4.5 1,200	510	128	165	2.40	3.60	194^a	2.18	19.6	1.76	11.2	83.0	55.8^a
Statistical variance
SEM	33.1	5.1	5.2	0.245	0.389	10.4	0.221	1.46	0.092	1.15	3.18	2.57
P-values
Ca	0.008	0.391	0.002	0.391	0.348	0.005	0.943	0.055	0.201	0.166	0.054	0.001
VD₃	0.901	0.817	0.658	0.599	0.971	0.166	0.832	0.404	0.197	0.123	0.201	0.001
Interaction	0.097	0.178	0.318	0.289	0.574	0.016	0.428	0.743	0.255	0.389	0.786	0.001

^a,b,c

Means in the same column with different superscripts are significantly different (P < 0.05). SEM, standard error of the means; IgG, immunoglobulin G; IgM, immunoglobulin M; IgA, immunoglobulin A; HIAI, hemagglutination-inhibition test for avian influenza; HIND, hemagglutination-inhibition test for Newcastle disease virus; PTH, parathyroid hormone; MDA, malondialdehyde; TAC, total antioxidant capacity; SOD, superoxide dismutase; CAT, catalase.