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. Author manuscript; available in PMC: 2020 Sep 17.
Published in final edited form as: Anal Chem. 2019 Aug 23;91(18):11794–11802. doi: 10.1021/acs.analchem.9b02411

Figure 2.

Figure 2.

MS spectra of (a) CH2DI permethylated fucosylated glycan, (b) 13CH3I permethylated fucosylated glycan, (c) CH2DI permethylated trisialylated triantennary glycan, and (d) 13CH3I permethylated trisialylated triantennary glycan. The glycans were released from model glycoprotein mixture (RNaseB, fetuin, and IgG), 8-plex permethylated and mixing at a ratio of 1:1:1:1:1:1:1:1 before analysis on LC-MS. Peaks with astarlikes indicate impurity peaks influenced by isotopic labeling iodomethane reagents purity. Symbols as in Table 1.