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. Author manuscript; available in PMC: 2020 Sep 17.
Published in final edited form as: Anal Chem. 2019 Aug 23;91(18):11794–11802. doi: 10.1021/acs.analchem.9b02411

Table 1.

Mass differences among isotopically permethylated glycans and MS resolutions needed to obtain a baseline mass separation.

graphic file with name nihms-1615369-t0002.jpg graphic file with name nihms-1615369-t0003.jpg graphic file with name nihms-1615369-t0004.jpg graphic file with name nihms-1615369-t0005.jpg
Number of permethylation sites 24 26 29 51
M[CH2D-glycan] 1596.9754 1855.1302 2062.2488 3647.1446
M[13CH3-glycan] 1596.9051 1855.0540 2062.1639 3646.9957
ΔM 0.0703 0.0761 0.0849 0.1489
M[CHD2-glycan] 1621.1260 1881.2933 2091.4308 3698.4647
M[13CH2D-glycan] 1621.0557 1881.2172 2091.3459 3698.3157
ΔM 0.0703 0.0761 0.0849 0.1489
M[CD3-glycan] 1645.2766 1907.4565 2120.6127 3749.7847
M[13CHD2-glycan] 1645.2063 1907.3803 2120.5279 3749.6358
ΔM 0.0703 0.0761 0.0849 0.1489
MS resolution 23,404 25,065 24,978 25,183

Symbols: Inline graphic, N-acetylglucosamine (GlcNAc); Inline graphic, Galactose (Gal); Inline graphic, Fucose (Fuc); Inline graphic, Mannose (Man); Inline graphic, N-acetylneuraminic acid (NeuAc/Sialic Acid).