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. 2020 Jul 31;11:1709. doi: 10.3389/fimmu.2020.01709

Figure 1.

Figure 1

Luciferase activities in 293T cells transfected with NFκB reporter plasmid. 293T cells were transiently transfected with pGL4.32 expressing firefly luciferase and pGL4.74 expressing renilla luciferase. (A,B) Cells were immediately transfected with the expression vectors of GRAs and ROPs, and the empty p3×FLAG-cmv14 vector used as a negative (empty) control. The promoter activity was determined and is shown as a fold-increase in the luciferase activity normalized for Renilla luciferase activity. (C) Pru, PruΔgra7 (deltaGRA7), PruΔgra14 (deltaGRA14), or PruΔgra15 (deltaGRA15) lines were added to the cells. After 12 h, parasites were added to the host cells, lysates were prepared, and luciferase activity was measured. The promoter activity was determined and is shown as a fold-increase in the luciferase activity normalized for Renilla luciferase activity. Values are the means ± SD of triplicate samples, *p < 0.05. #a significant difference with the control vector and or uninfected cells (p < 0.05). Differences were tested by one-way ANOVA with turkey's post-hoc test in (B,C). Data are representative of two independent experiments.