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. 2020 Jul 31;11:1709. doi: 10.3389/fimmu.2020.01709

Figure 2.

Figure 2

GRA expression activates nuclear translocation of NF-κB RelA in 293T cells. (A) 293T cells were transfected with the expression vectors for GRA7, GRA14, or GRA15, or the empty p3 × FLAG-cmv14 vector used as a negative (empty) control. Cells were then fixed and stained with α-NFκB RelA (red), α-FLAG (green), or Hoechst dye (blue). Bars, 10 μm. (B) The mean intensity of RelA in the nucleus was measured for at least 20 cells per group. Bar indicates the mean of each group, *p < 0.05. #a significantly higher level of nuclear RelA compared with the control cells (p < 0.05). Differences were tested by one-way ANOVA with turkey's post-hoc test. Experiments were performed twice.