Figure 3.

T. gondii infection with parasites deficient in GRA7, 14, or 15 decreases nuclear translocation of NFκB RelA in HFF cells. (A–C) The mean intensity of RelA in the nucleus was measured in HFF cells for each group. Experiments were performed twice. Bar indicates the mean of each group, *p < 0.05. #a significantly higher level of nuclear RelA compared with uninfected cells (p < 0.05). Differences were tested by one-way ANOVA with turkey's post-hoc test. (D) Representative IFAT images of HFF cells infected with parasite strains and uninfected control cells. After infection for 24 h, cells were fixed and stained with α-NFκB RelA (red), α-SAG1 (green), and Hoechst dye (blue). Bars, 10 μm.