Figure 6.

GRA deficiency resulted in downregulation of gene expression mediated by NFκB. RNA-seq analysis of Raw246.7 macrophage cells infected for 24 h with parasite strains and the uninfected cells (Mock) (n = 1 per group). DESeq analysis identified genes with a more than 2-fold change in expression between the GRA deficient strain and the parental Pru and complemented strains following infection. (A) Venn diagrams comparing GRA7, GRA14, and GRA15-dependent DEGs during T. gondii infection: down, downregulated; up, upregulated. (B) Heatmap showing that the most significantly enriched pathway associated with the GRA14 expression status was the cytokine-cytokine receptor interaction, which contained a subset of 41 genes. Rows represent samples from different processes, and columns represent genes. (C) Heatmap showing that the most significantly enriched pathway associated with the GRA7 expression status was the IL17 signaling pathway, which contained a subset of 91 genes. Rows represent samples from different processes, and columns represent genes. The cluster shown in (B,C), which was upregulated when GRA14 or GRA7 expression was restored, is enlarged on the right. The experiment for GRA14 was performed in tandem with GRA15, and the experiment for GRA7 was performed independently.