Table 1.
Plasmids used in this study.
| Plasmid | Description | Use | Source or reference |
|---|---|---|---|
| pBS/GFP/HX | Plasmid for cloning TgGRA7 knock-out vector | This study | |
| pBS/GFP/TgGRA7KO/HX | HXGPRT cassette flaked by two homology arms from the 5'- and 3'- UTR of TgGRA7 gene | The knock-out vector targeting TgGRA7 gene | This study |
| pSAG1::CAS9-U6::sgUPRT | CAS9 expressed from the ToxoplasmaSAG1 promoter and CRISPR gRNA targeting Toxoplasma UPRT produced from the U6 promoter | CRISPR plasmid targeting Toxoplasma UPRT | Addgene |
| pSAG1::CAS9-U6::sgTgGRA14 | CAS9 expressed from the TgSAG1 promoter and CRISPR gRNA targeting TgGRA14 produced from the U6 promoter | CRISPR plasmid targeting between nucleotides 488 and 489 in TgGRA14 gene | This study |
| pSAG1::CAS9-U6::sgTgGRA15 | CAS9 expressed from the TgSAG1 promoter and CRISPR gRNA targeting TgGRA15 produced from the U6 promoter | CRISPR plasmid targeting between nucleotides 146 and 147 in TgGRA14 gene | This study |
| pUPRT::DHFR-D | DHFR* cassette flanked by two homology arms from the 5′- and 3′-UTR of UPRT gene, respectively | Knockin the DHFR* expressed cassette into targeting gene | Addgene |
| p3XFLAG-CMV-14 | Plasmid for cloning of FLAG tag fused gene | Sigma-Aldrich | |
| p3XFLAG-CMV-TgGRA1 | FLAG tag-fused Tg(GOI) | Luciferase reporter assay | This study |
| p3XFLAG-CMV-TgGRA2 | This study | ||
| p3XFLAG-CMV-TgGRA3 | This study | ||
| p3XFLAG-CMV-TgGRA4 | This study | ||
| p3XFLAG-CMV-TgGRA5 | This study | ||
| p3XFLAG-CMV-TgGRA6 | This study | ||
| p3XFLAG-CMV-TgGRA7 | This study | ||
| p3XFLAG-CMV-TgGRA8 | This study | ||
| p3XFLAG-CMV-TgGRA9 | This study | ||
| p3XFLAG-CMV-TgGRA11 | This study | ||
| p3XFLAG-CMV-TgGRA12 | This study | ||
| p3XFLAG-CMV-TgGRA14 | This study | ||
| p3XFLAG-CMV-TgGRA15 | This study | ||
| p3XFLAG-CMV-TgGRA16 | This study | ||
| p3XFLAG-CMV-TgGRA23 | This study | ||
| p3XFLAG-CMV-TgGRA24 | This study | ||
| p3XFLAG-CMV-TgGRA25 | This study | ||
| p3XFLAG-CMV-TgROP5 | This study | ||
| p3XFLAG-CMV-TgROP8 | This study | ||
| p3XFLAG-CMV-TgROP9 | This study | ||
| p3XFLAG-CMV-TgROP10 | This study | ||
| p3XFLAG-CMV-TgROP11 | This study | ||
| p3XFLAG-CMV-TgROP12 | This study | ||
| p3XFLAG-CMV-TgROP13 | This study | ||
| p3XFLAG-CMV-TgROP14 | This study | ||
| p3XFLAG-CMV-TgROP15 | This study | ||
| p3XFLAG-CMV-TgROP16 | This study | ||
| p3XFLAG-CMV-TgROP17 | This study | ||
| p3XFLAG-CMV-TgROP18 | This study | ||
| p3XFLAG-CMV-TgROP19A | This study | ||
| p3XFLAG-CMV-TgROP20 | This study | ||
| p3XFLAG-CMV-TgROP23 | This study | ||
| p3XFLAG-CMV-TgROP24 | This study | ||
| p3XFLAG-CMV-TgROP26 | This study | ||
| p3XFLAG-CMV-TgROP34 | This study | ||
| p3XFLAG-CMV-TgROP35 | This study | ||
| p3XFLAG-CMV-TgROP38 | This study | ||
| p3XFLAG-CMV-TgROP39 | This study | ||
| pGL4.32 | Nuclear factor-κB response element (NF-κB) | Luciferase reporter assay for NF-κB signal | Promega |
| pGL4.74 | Control Renillaluciferase expression vector | Promega | |
| pBluescript SK (+) | Plasmid for cloning of TgGRA14+UTR gene | Add gene | |
| pBluescript SK (+)-TgGRA7+UTR | TgGRA14 expressed from the TgGRA7 5′UTR and 3′ UTR | Replacing the UPRT gene by TgGRA7 | This study |
| pBluescript SK (+)-TgGRA14+UTR | TgGRA14 expressed from the TgGRA14 5′UTR and 3′ UTR | Replacing the UPRT gene by TgGRA14 | This study |