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. 2020 Jul 31;11:911. doi: 10.3389/fphys.2020.00911

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Copyright © 2020 Luo, Wang, Ding, Hasan, Yang, Lin, Schreppel, Sun, Yin, Erker and Sun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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STS66 reduced activation of WNK1-SPAK/OSR1-NKCC1 cascade in cultured glioma cells in response to TMZ. (A) Representative immunoblotting images of total (t-) or phosphorylated (p-) WNK1, SPAK/OSR1, and NKCC1. GL26 cells were exposed to DMSO (Veh), TMZ (100 μM), BMT (10 μM), STS66 (10 μM), T + B, or T + S combined for 48 h. Cell lysates were harvested for immunoblotting. (B) Summary. Data are means ± SEM (n = 4, 5), ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001. (C) Representative immunoblotting images of total (t-) or phosphorylated (p-) WNK1, SPAK/OSR1, and NKCC1. SB28-GFP cells were exposed to DMSO (Veh), TMZ (100 μM), BMT (10 μM), STS66 (10 μM), T + B, or T + S combined for 48 h. (D) Summary. Data are means ± SEM (n = 4, 5), ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001.