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VB1 is linked with modified gold nanoparticles for further HPLC and immunoprecipitation. (A) The chemical structure diagram of VB1. (B) The gold nanoparticles were synthesized and shown in the transmission electron microscope (TEM) image. (C) Gold nanoparticles was modified by lignin via reacting with 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC, 100 mM) and N-hydroxysuccinimide (NHS, 100 mM).
