FIGURE 5.

Topical VB1 gel palliates skin photoaging phenotype induced by UVA irradiation in mice. Mice were irradiated with UVA 3 times per week at doses of 20 J/cm² for 12 weeks. A gel containing 2% VB1 or a vehicle gel lacking VB1 (negative control) was applied topically to the dorsal skin areas of mice at 1 h after each irradiation. The control group consisted of untreated mice at the same age. (A) Representative photographs of mice dorsal skin in mice from control group, UVA-irradiated group, the experimental group receiving VB1 gel and the vehicle only group. (B) Representative photographs of hematoxylin-eosin (HE)-stained dorsal skin sections obtained at the end of the experiment from (a) control group, (b) UVA-irradiated group, (c) the experimental group receiving VB1 gel and (d) the vehicle only group (left panel, magnification 200x). Epidermal thicknesses were estimated at 5 different random sites with each mouse from digital images of HE-stained sections and are depicted as means ± SEM (n = 10 per group). The right panel showed the analysis data (+ vs. ctrl, p < 0.05, * vs. UVA, p < 0.05).