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. 2020 Jul 31;8:905. doi: 10.3389/fbioe.2020.00905

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Copyright © 2020 Sun, Lu, Liang, Yang and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Construction of an efficient cytosine base editing system in Pseudomonas putida KT2440. (A) Six different modules for cytosine base editing designed in this study. (B) Determination of pSEVA-Module 6 (pSEVA6BE) mediated cytosine base editing in the TtgA spacer region. (C) Potential off-target loci of TtgA in P. putida KT2440 genome.